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CRC cells promote own growth and expansion

Mon, 07/12/2021 - 18:04
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Through a complex, self-reinforcing feedback mechanism, colorectal cancer cells make room for their own expansion by driving surrounding healthy intestinal cells to death, while simultaneously fuelling their own growth. This feedback loop is driven by an activator of the innate immune system, according to researchers from the German Cancer Research Center (DKFZ) and the University of Heidelberg, who discovered this mechanism in the intestinal tissue of fruit flies. They reporte their findings in the paper, ‘Microenvironmental innate immune signaling and cell mechanical responses promote tumor growth’, published in the journal Developmental Cell.

Maintaining the well-functioning state of an organ or tissue requires a balance of cell growth and differentiation on the one hand, and the elimination of defective cells on the other. The intestinal epithelium is a well-studied example of this balance, termed 'tissue homeostasis': Stem cells in the intestinal crypts constantly produce progenitor cells that further differentiate to replace the rapidly deteriorating mature cells of the intestinal mucosa.

Growth requires a constant dynamic reorganization of tissue architecture: Defective cells must be displaced from the tissue, also by mechanical forces. Tumour cells disrupt this finely balanced structure: They aggressively make room for their own expansion. Until now, it was not understood how exactly they do this.

Jun Zhou, Erica Valentini and Michael Boutros from the German Cancer Research Center and the University of Heidelberg have now investigated these processes in the intestinal epithelium of the fruit fly, which has a similar structure to the intestine of mammals. By blocking the important BMP signalling pathway, the researchers triggered numerous tumours in the fly intestine. Using this model, they uncovered how cancer cells accelerate their own growth through a sophisticated, self-reinforcing feedback effect.

First, tumour cells tear apart the tissue structure by acting on cell adhesion. The resulting altered mechanical adhesion of the intestinal cells activates a stress-sensitive signalling pathway in neighbouring intestinal cells, which in turn causes the activation og genes that promote programmed cell death (apoptosis): The team was able to detect large amounts of proteins that initiate aopotosis in the intestinal cells surrounding individual tumour cells.

Presumably due to cytokines released by dying intestinal cells, the growth-promoting JAK/Stat signalling pathway is activated in tumour cells, leading to further tumour spread. The team also found that the immune activator PGRP-LA is required for this process. When PGRP-LA is turned off, fewer colon cells die through apoptosis and tumour growth also slows down.

"The colon cancer cells apparently hijack a signalling molecule of the innate immune system and misuse it for their own purposes. In this way, they kill two birds with one stone: they make room for their expansion by eliminating intestinal cells in their environment and additionally fuel their own growth," explained study leader Michael Boutros. Future studies will determine whether the same mechanisms also play a role in the spread of human intestinal tumours.”